Top 5 Things To Know While Selecting Elisa Kits 

The ELISA technique is a powerful way to detect low-level proteins, hormones, or other analytes in samples. It has been common in industry and academia for many years. ELISA uses antibodies that are highly specific to the analyte assayed. 

The ELISA process is a step-by-step method used by many researchers. It tests and proves their hypotheses of the presence or absence of specific substances in biological samples such as blood, saliva, and urine. There is a need to invest in high-quality kits to avoid errors with your tests. 

ELISA combines its accuracy and convenience to provide you with the most reliable results for your tests. There is every reason to pick it for your needs. But do you know what it takes to find the most appropriate kit for your samples? 

When selecting an efficient ELISA kit, you might consider the cost, ease of use, sensitivity, detection limit, and linearity range. But that is not all. In this post, we will discuss five significant points for consideration when choosing an ELISA kit. Read on to learn how to pick the most appropriate ELISA kit in five simple steps. 

  1. Consider the Species

 

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Not all ELISA kits work for all samples. The kits contain reagents that target specific proteins from a closely-related variety of species. If you use different samples from what the ELISA kit is prepared, you may have trouble detecting them. 

For some classical species, it is easier to find the kits. However, for others, there may not be any kits available. Ensure that you purchase from trusted ELISA kit manufacturers like Sigma-Aldrich, Abcam, Biolegend, and LifeSpan BioSciences. 

In such cases, you will better use homology in determining the closest species that can match the samples. That is possible through the use of multiple sequence alignments. 

  1. Type of Antibody to Detect

 

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There are various types of antibodies that ELISA kits can detect, ranging from monoclonal to polyclonal. You may not get accurate results by using a monoclonal antibody kit with polyclonal assays. 

Also, you must understand that monoclonal antibodies are best suited for detection. The polyclonal antibodies work best for capture. 

Another thing to consider when choosing an ELISA kit is whether or not it is validated for your samples. Antibody validation is significant in research. You can check the validity of the kits by reading product information on the company’s website. 

  1. Size of the Sample

 
 

Another significant factor to consider when selecting an ELISA kit is the sample size. Analyzing a small sample will require you to use less reagent, which means the kit will be cost-effective. 

You need to understand that using fewer samples can save your time and money in the long run because it shortens the assay procedure. You should aim for a kit that requires tiny amounts of the sample if you are testing for very precious or rare proteins. 

The concentration of the samples also determines the sensitivity of the kit you will buy. Remember that low concentrations will require high sensitivity. As such, you would have to pay more for it. 

 

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  1. Linearity and Recovery of the Tests

 
 

There is a need for consistency when testing your samples, regardless of the condition and concentration. Therefore, you will have to select a kit that gives the best results consistently. Also, look for kits with high recovery rates to not affect your findings. The percentage of recovered proteins is vital when comparing two different ELISA tests. 

Linearity is the measure of how consistent the results remain when you dilute the samples. A viable ELISA kit should always show consistency. 

A high-quality ELISA kit will give you accurate and precise results, which is why it’s advisable to look for one with low intra-assay variability. You will ensure that your findings are always valid. 

  1. Your Budget

 

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In every purchase, the cost of the item matters a lot. Buying ELISA kits is more or less the same. After determining the technical aspects of the kits match your expectations, you can consider your budget to narrow down your options. Some kits are highly-priced because they offer additional features that other brands do not. 

However, do not use pricing as a basis for your ultimate choice. Cheap products may not last long. They could also mean poor validation or none at all. Shopping around from various manufacturers is a smart way to find the best ELISA kits at an affordable price. 

Also, ensure you understand what is in the pricing of the kits. Some companies charge separately for the manual and other items such as peptides or adhesives. If you have to buy separate things, it can get costly very quickly. 

Finally, remember that ELISA kits are consumables and will expire after some time or use. You want them to last long enough to take you through the study without affecting performance. However, sometimes they reach expiry before you even open them up for testing. 

It is paramount to check the expiration date of the kit when purchasing new ones. After reaching its expiry date, there is nothing you can do to rescue it. Avoid buying expired items trying to save money. 

Conclusion 
 

While ELISA can give you more accurate results, you need to find one that matches your needs. The process can be time-consuming, especially when you are new to the field. Just be patient and diligent in your search for a good kit. You can also ask your peers for suggestions. 

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